INHIBITION AND ENHANCEMENT OF EUKARYOTIC GENE-EXPRESSION BY POTENTIAL NON-B DNA-SEQUENCES

被引：14

作者：

DELIC, J

ONCLERCQ, R

MOISANCOPPEY, M

机构：

[1] INST PASTEUR,UNITE GENET MAMMIFERES,F-75724 PARIS 15,FRANCE

[2] LAB RADIOPATHOL,MED SECT,F-75005 PARIS,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 181卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91263-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In a transient or constitutive expression assay we have examined the effect of non-B DNA sequences d(CA)40 and d(CAAAAATGCC)n on gene expression in eukaryotic cells. These sequences were cloned adjacent to the weak eukaryotic promoter (CGTATTTATTTG) and located upstream from the coding sequence of galactokinase enzyme. Recombinants were micro-injected in cultured cells (Chinese hamster fibroblasts R1610, mutant gal-K-) and expression levels have been determined. The alternating purine-pyrimidine tract found in d(CA)40 able to assume the Z-DNA conformation shows an inhibitory effect on gene expression. In addition, our results suggest a new potential role of Z-DNA motifs in vivo to stimulate recombination. The sequences d(CAAAAATGCC)n able to adopt another non-B structure, corresponding to curved (or bended) helix conformation, strongly enhance gene expression and this enhancement depends on sequence redundancy. © 1991.

引用

页码：818 / 826

页数：9

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