GENERATION OF ANGIOTENSIN-II FROM HUMAN PLASMA BY TISSUE KALLIKREIN

被引：12

作者：

KRIVOY, N

SCHULTER, H

KARAS, M

ZIDEK, W

机构：

[1] UNIV MUNSTER,MED POLIKLIN,ALBERT SCHWEITZER STR 33,W-4400 MUNSTER,GERMANY

[2] UNIV MUNSTER,MED PHYS,W-4400 MUNSTER,GERMANY

[3] RAMBAM MED CTR,MED A & CLIN PHARMACOL UNIT,HAIFA,ISRAEL

[4] B RAPPAPORT FAC MED,HAIFA,ISRAEL

来源：

CLINICAL SCIENCE | 1992年 / 83卷 / 04期

关键词：

ANGIOTENSIN-II; PLASMA; TISSUE KALLIKREIN;

D O I：

10.1042/cs0830477

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

1. Human plasma was incubated with tissue kallikrein from porcine pancreas, dialysed to obtain a fraction with a molecular mass<10 kDa and further purified by reverse-phase chromatography. 2. Vasopressor activity in the fractions obtained was tested in the isolated perfused rat kidney. 3. In one fraction a strong vasopressor action was found, which was blocked by saralasin and by an angiotensin II antibody. 4. Aprotinin inhibited the formation of vasopressor substances by tissue kallikrein. 5. U.v.-laser desorption/ionization mass spectrometry revealed a molecular mass of 1046 Da in the purified active fraction. 6. It is concluded that tissue kallikrein forms not only kinins, but also angiotensin II, from human plasma under physiological conditions.

引用

页码：477 / 482

页数：6

共 12 条

[1] ABILITY OF KALLIKREIN TO GENERATE ANGIOTENSIN-II-LIKE PRESSOR SUBSTANCE AND A PROPOSED KININ-TENSIN ENZYME-SYSTEM [J].