PULSATILE FLUCTUATIONS OF PLASMA-GONADOTROPIN-RELEASING HORMONE AND CORTICOTROPIN-RELEASING FACTOR LEVELS IN HEALTHY PREGNANT-WOMEN

Several data emphasize the neuroendocrine aspects of human placenta. Classical hypophyseotropic neurohormones are produced and secreted by the human placenta. Indeed, in contrast with non pregnant subjects, gonadotropin-releasing hormone (GnRH) and coritcotropin-releasing factor (CRF) are measurable in maternal plasma during pregnancy. The aim of the present study was to investigate the characteristics of the secretory pattern of maternal plasma GnRH and CRF levels. A total of 25 healthy pregnant women participated in the present cross-sectional study. Plasma levels of the two neurohormones were measured according to three different time-intervals: 1) every five minutes for eight hours (n = 4), 2) every ten minutes for four hours (n = 15), 3) every four hours for 24 hours (n = 7). Following an acidic extraction plasma GnRH and CRF levels were measured by specific radioimmunoassay. Using two different algorithms (Detect and Cluster) a pulsatile pattern for both plasma GnRH and CRF levels was observed. Specific pulse frequency, amplitude, and duration were found. In the groups of women studied with a longer sampling interval, pulse frequency of GnRH or CRF levels did not differ between first and third trimester of gestation. However, amplitude of CRF pulses were significantly higher at term than at early stages of pregnancy (p <0.01), while GnRH pulse amplitude was highest in women at first trimester of gestation. Evaluating the degree of concordance in all subjects, GnRH pulses significantly correlated with CRF pulses at 30 min. lag (p<0.01). No significant circadian changes were found for any circulating neurohormone measured. The present study showed that maternal circulating neurohormone levels change according to a pulsatile pattern, probably reflecting the placental secretory events.