MULTIPLE AGE-ASSOCIATED MITOCHONDRIAL-DNA DELETIONS IN SKELETAL-MUSCLE OF MICE

被引：57

作者：

CHUNG, SS ^{[1
]}

WEINDRUCH, R ^{[1
]}

SCHWARZE, SR ^{[1
]}

MCKENZIE, DI ^{[1
]}

AIKEN, JM ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT ANIM HLTH & BIOMED SCI,MADISON,WI 53706

来源：

AGING-CLINICAL AND EXPERIMENTAL RESEARCH | 1994年 / 6卷 / 03期

关键词：

AGING; MICE; MITOCHONDRIAL DNA DELETIONS;

D O I：

10.1007/BF03324239

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

Multiple mitochondrial DNA (mtDNA) deletions have been associated with aging in humans and monkeys. Since the inbred mouse strain, C57BL/6, hers been extensively studied gerontologically, we sought to investigate its utility as a model for examining the importance of mtDNA deletions in aging. Using the polymerase chain reaction (PCR), vie analyzed hind limb skeletal muscle from mice of three age groups (5, 16 and 25 months) for the presence of age-associated mtDNA deletions. We observed multiple mtDNA deletions in all three age groups. Further, the number of deletions detected per mouse increased greatly tuith advancing age.

引用

页码：193 / 200

页数：8

共 32 条

[1]

Linnane A.W., Marzuki S., Ozawa T., Tanaka M., Mitochondrial DNA mutations as an important contributor to ageing and degenerative diseases, Lancet, 1, pp. 642-645, (1989)

[2]

Cortopassi G.A., Arnheim N., Detection of a specific mitochondrial DNA deletion in tissues of older humans, Nucleic Acids Res., 18, pp. 6927-6933, (1990)

[3]

Hattori K., Ogawa T., Kondo T., Mochizuki M., Tanaka M., Sugiyama S., Ito T., Satake T., Ozawa T., Cardiomyopathy with mitochondrial DNA mutations, Am. Heart J., 122, pp. 866-869, (1991)

[4]

Wallace D.C., Mitochondrial genetics: A paradigm for aging and degenerative diseases?, Science, 256, pp. 628-632, (1992)

[5]

Yen T.C., Su J.H., King K.L., Wei Y.H., Ageing-associated 5 kb deletion in human liver mitochondrial DNA, Biochem. Biophys. Res. Comm., 178, pp. 124-131, (1991)

[6]

Hattori K., Tanaka M., Sugiyama S., Obayashi T., Ito T., Satake T., Hanaki Y., Asai J., Nagano M., Ozawa T., Age-dependent increase in deleted mitochondrial DNA in the human heart: Possible contributory factor to presbycardia, Am. Heart J., 121, pp. 1735-1742, (1991)

[7]

Corral-Debrinski M., Horton T., Lott M.T., Shoffner J.M., Beal M.F., Wallace D.C., Mitochondrial DNA deletions in human brain: regional variability and increase with advanced age, Nature Genetics, 2, pp. 324-329, (1992)

[8]

Cortopassi G.A., Shibata D., Soong N.W., Arnheim N., A pattern of accumulation of a somatic deletion of mitochondrial DNA in aging human tissues, Proc. Natl. Acad. Sci. USA, 89, pp. 7370-7374, (1992)

[9]

Yen T.C., Pang C.Y., Hsieh R.H., Su C.H., King K.L., Wei Y.H., Age-dependent 6 kb deletion in human liver mitochondrial DNA, Biochem. Int., 26, pp. 457-468, (1992)

[10]

Gadaleta M.N., Rainaldi G., Lezza A., Milella F., Fracasso F., Cantatore P., Mitochondrial DNA copy number and mitochondrial DNA deletions in adult and senescent rats, Mutat. Res., 275, pp. 181-193, (1992)

← 1 2 3 4 →