ANTIGENIC PROPERTIES OF PROTEINS CROSS-LINKED BY MULTIDIAZONIUM COMPOUNDS

The antigenic properties of cross-linked proteins were examined. Cross-linking was performed by coupling proteins to the diazonium groups of a mixed polycondensate of aromatic amino carboxylic acids. The resulting cross-linked protein samples were soluble under physiological conditions. The immunogenic capacity was determined without the use of adjuvants by intravenous injection. The cross-linked proteins have been found to give a greatly enhanced antibody response compared with the original proteins. The antisera obtained after immunization with cross-linked proteins cross-reacted with the original protein monomers (kallikrein inactivator, ribonuclease, human chorionic gonadotropin) and neutralized their biological activity. © 1969.