MEASUREMENT OF 5-METHYLTETRAHYDROFOLIC ACID IN MAN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引:36
作者
LEEMING, RJ [1 ]
POLLOCK, A [1 ]
MELVILLE, LJ [1 ]
HAMON, CGB [1 ]
机构
[1] UNIV ASTON,DIV BIOL,BIRMINGHAM B4 7ET,W MIDLANDS,ENGLAND
来源
METABOLISM-CLINICAL AND EXPERIMENTAL | 1990年 / 39卷 / 09期
关键词
D O I
10.1016/0026-0495(90)90298-Q
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
5-Methyl-5,6,7,8-tetrahydrofolic (5-methylTHF) acid can be measured in human serum and whole blood by fluorescence after deproteinization with perchloric acid and separation by high-performance liquid chromatography (HPLC). The technique is sensitive, rapid, and correlates with existing methods for total folates. © 1990.
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页码:902 / 904
页数:3
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