METABOLIC REGULATION OF POLYGALACTURONIC ACID TRANS-ELIMINASE IN ERWINIA

被引:43
作者
MORAN, F
STARR, MP
机构
[1] Department of Bacteriology, University of California, Davis, California
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 11卷 / 02期
关键词
D O I
10.1111/j.1432-1033.1969.tb00772.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Strains of Erwinia carotovora and Erwinia aroideae, formerly thought only to be inducible, are constitutive for polygalacturonic acid trans‐eliminase. Maximum enzyme formation, however, occurs in the presence of polygalacturonic acid. A release from strong catabolic repression enables glucose‐grown cultures to initiate their attack on polygalacturonic acid and, thereby, eliminates the necessity for the entry of the polymer into the cell. A low constant proportion (less than 25%) of the total polygalacturonic acid trans‐eliminase is present in the culture fluid during the exponential growth‐phase of Erwinia carotovora. The enzyme may be regarded as an exoenzyme. The exact location of polygalacturonic acid trans‐eliminase in the cells was not established; it could be truly intracellular or reside between the cell‐wall and membrane. Copyright © 1969, Wiley Blackwell. All rights reserved
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页码:291 / &
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