ONE-STEP TRANSFORMATION OF YEAST IN STATIONARY PHASE

被引：625

作者：

CHEN, DC

YANG, BC

KUO, TT

机构：

[1] NATL TAIWAN UNIV, GRAD INST BOT, TAIPEI 115, TAIWAN

[2] ACAD SINICA, INST BOT, TAIPEI 115, TAIWAN

[3] ACAD SINICA, INST MOL BIOL, TAIPEI, TAIWAN

来源：

CURRENT GENETICS | 1992年 / 21卷 / 01期

关键词：

YEAST; TRANSFORMATION; THIO COMPOUND; STATIONARY PHASE;

D O I：

10.1007/BF00318659

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45-degrees-C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5 x 10(8) cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 10(4) transformants/mu-g plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

引用

页码：83 / 84

页数：2

共 14 条

[1] BRZOBOHATY B, 1986, J GEN MICROBIOL, V132, P3089
[2] TRANSFORMATION OF YEAST
HINNEN, A
HICKS, JB
FINK, GR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (04) : 1929 - 1933
[3] TRANSFORMATION OF INTACT YEAST-CELLS TREATED WITH ALKALI CATIONS
ITO, H
FUKUDA, Y
MURATA, K
KIMURA, A
[J]. JOURNAL OF BACTERIOLOGY, 1983, 153 (01) : 163 - 168
[4] TRANSFORMATION OF YEAST-CELLS TREATED WITH 2-MERCAPTOETHANOL
ITO, H
MURATA, K
KIMURA, A
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1983, 47 (07): : 1691 - 1692
[5] TRANSFORMATION OF INTACT YEAST-CELLS TREATED WITH ALKALI CATIONS OR THIOL COMPOUNDS
ITO, H
MURATA, K
KIMURA, A
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1984, 48 (02): : 341 - 347
[6] JONES EW, 1977, GENETICS, V85, P23
[7] KATOHDA S, 1976, PLANT CELL PHYSIOL, V17, P909
[8] A PROTEASE THAT PARTICIPATES IN YEAST-CELL WALL LYSIS DURING ZYMOLYASE DIGESTION
KITAMURA, K
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1982, 46 (08): : 2093 - 2099
[9] A GENERAL-METHOD FOR POLYETHYLENE-GLYCOL-INDUCED GENETIC-TRANSFORMATION OF BACTERIA AND YEAST
KLEBE, RJ
HARRISS, JV
SHARP, ZD
DOUGLAS, MG
[J]. GENE, 1983, 25 (2-3) : 333 - 341
[10] HIGH-EFFICIENCY TRANSFORMATION OF INTACT YEAST-CELLS BY ELECTRIC-FIELD PULSES
MEILHOC, E
MASSON, JM
TEISSIE, J
[J]. BIO-TECHNOLOGY, 1990, 8 (03): : 223 - 227

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