PHYSIOLOGICAL FACTORS AFFECTING CARBON-TETRACHLORIDE DEHALOGENATION BY THE DENITRIFYING BACTERIUM PSEUDOMONAS SP STRAIN KC

被引:37
作者
LEWIS, TA
CRAWFORD, RL
机构
[1] UNIV IDAHO,DEPT BACTERIOL & BIOCHEM,MOSCOW,ID 83843
[2] UNIV IDAHO,CTR HAZARDOUS WASTE REMEDIAT RES,MOSCOW,ID 83843
关键词
D O I
10.1128/AEM.59.5.1635-1641.1993
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Pseudomonas sp. strain KC was grown on a medium with a low content of transition metals in order to examine the conditions for carbon tetrachloride (CT) transformation. Several carbon sources, including acetate, glucose, glycerol, and glutamate, were able to support CT transformation. The chelators 2,2'-dipyridyl and 1,10-phenanthroline stimulated CT transformation in a rich medium that otherwise did not support this activity. Low (< 10 muM) additions of dissolved iron(II), iron(III), and cobalt(II), as well as an insoluble iron(III) compound, ferric oxyhydroxide, inhibited CT transformation. The addition of 50 muM iron to actively growing cultures resulted in delayed inhibition of CT transformation. CT transformation was seen in aerobic cultures of KC, but with reduced efficiency compared with denitrifying cultures. Inhibition of CT transformation by iron was also seen in aerobically grown cultures. Optimal conditions were used in searching for effective CT transformation activity among denitrifying enrichments grown from samples of aquifer material. No activity comparable to that of Pseudomonas sp. strain KC was found among 16 samples tested.
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页码:1635 / 1641
页数:7
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