THE CHITINASE ENCODING TN7-BASED CHIA GENE ENDOWS PSEUDOMONAS-FLUORESCENS WITH THE CAPACITY TO CONTROL PLANT-PATHOGENS IN SOIL

被引：16

作者：

KOBY, S

SCHICKLER, H

CHET, I

OPPENHEIM, AB

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH MED SCH,DEPT MOLEC GENET,IL-90101 JERUSALEM,ISRAEL

[2] HEBREW UNIV JERUSALEM,FAC AGR,OTTO WARBURG CTR AGR BIOTECHNOL,IL-76100 REHOVOT,ISRAEL

来源：

GENE | 1994年 / 147卷 / 01期

关键词：

TRANSPOSON; RECOMBINANT DNA; SERRATIA; RHIZOCTONIA; P-TAC PROMOTER;

D O I：

10.1016/0378-1119(94)90042-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A transposon system based on Tn7 carrying the chiA gene (encoding chitinase) of Serratia marcescens was established. The transposon was introduced by conjugation into Pseudomonas fluorescens as a stable integrate expressing and secreting the active chitinase enzyme. The resulting strain was shown to control the plant pathogen Rhizoctonia solani in soil.

引用

页码：81 / 83

页数：3

共 8 条

[1] AN IMPROVED TN7-BASED SYSTEM FOR THE SINGLE-COPY INSERTION OF CLONED GENES INTO CHROMOSOMES OF GRAM-NEGATIVE BACTERIA [J].