EFFECTS OF POTASSIUM CHANNEL BLOCKERS ON BASAL VASCULAR TONE AND REACTIVE HYPEREMIA OF CANINE DIAPHRAGM

被引:34
作者
VANELLI, G
HUSSAIN, SNA
机构
[1] ROYAL VICTORIA HOSP, DIV CRIT CARE, MONTREAL H3A 1A1, PQ, CANADA
[2] ROYAL VICTORIA HOSP, DIV RESP MED, MONTREAL H3A 1A1, PQ, CANADA
[3] MCGILL UNIV, MEAKINS CHRISTIE LABS, MONTREAL H3A 1A1, PQ, CANADA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1994年 / 266卷 / 01期
关键词
GLIBENCLAMIDE; IBERIOTOXIN; APAMIN; PHRENIC FLOW; RESPIRATORY MUSCLES;
D O I
10.1152/ajpheart.1994.266.1.H43
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Glibenclamide, iberiotoxin, and apamin (blockers of ATP-sensitive, large-conductance, and small-conductance Ca2+-activated potassium channels, respectively) were infused into the diaphragmatic vasculature of anesthetized dogs to assess the contribution of these channels in the regulation of basal tone and the response to brief occlusions of the left phrenic artery (reactive hyperemia). Baseline phrenic flow (Q(phr)), peak postocclusive flow, and reactive hyperemia duration in response to 10-, 30-, 60-, and 120-s arterial occlusions were measured before (control) and after the infusion of K+ channel blockers in three groups of animals. Glibenclamide at 5 x 10(-6), 1 x 10(-5), and 8 x 10(-5) M increased baseline phrenic resistance to 140, 204, and 192% of control values, respectively. Peak postocclusive Q(phr) and duration of hyperemia in response to all occlusion durations were significantly attenuated after glibenclamide infusion. Iberiotoxin infusion at 1 x 10(-8), 3 x 10(-8), and 1 x 10(-7) M increased phrenic resistance to 141, 133, and 146% of control values, respectively. By comparison, baseline phrenic resistance rose to 159 and 145% of control in response to 1 x 10(-7) and 1 x 10(-6) M apamin, respectively. Iberiotoxin and apamin reduced peak postocclusive flow and duration of hyperemia only in response to 10- and 30-s occlusions. We infused K+ channel blockers along with lemakalim into the diaphragm during constant flow perfusion in separate groups of animals. When infused alone, lemakalim reduced phrenic resistance by 60-70%. Glibenclamide dose dependently reversed lemakalim-induced vasodilation, whereas neither iberiotoxin nor apamin influenced this dilation. These results suggest that baseline resistance and reactive hyperemia of the diaphragm are regulated in part by the activities of potassium channels, particularly those sensitive to glibenclamide.
引用
收藏
页码:H43 / H51
页数:9
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