BORDETELLA-PERTUSSIS FIMBRIAE BIND TO HUMAN MONOCYTES VIA THE MINOR FIMBRIAL SUBUNIT FIMD

被引:28
作者
HAZENBOS, WLW
GEUIJEN, CAW
VANDENBERG, BM
MOOI, FR
VANFURTH, R
机构
[1] UNIV LEIDEN HOSP, DEPT INFECT DIS, 2300 RC LEIDEN, NETHERLANDS
[2] NATL INST PUBL HLTH & ENVIRONM PROTECT, MOLEC MICROBIOL UNIT, 3720 BA BILTHOVEN, NETHERLANDS
关键词
D O I
10.1093/infdis/171.4.924
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Nonopsonized Bordetella pertussis can bind to and become ingested by human monocytes. Previous studies demonstrated that mutant B. pertussis strains that lack fimbriae express a reduced adherence to monocytes. The present study was undertaken to investigate the involvement of the minor fimbrial subunit FimD in the adherence of B. pertussis to human monocytes using purified fimbriae, FimD, and strains with mutations in fimbrial genes. Flow cytometry demonstrated that purified B. pertussis fimbriae avidly bind to monocytes in a dose-dependent manner but bind less to neutrophils and hardly to lymphocytes; FimD-lacking fimbriae did not bind to monocytes. Purified fimbriae or FimD inhibited the binding of wild-type B. pertussis to monocytes in a dose-dependent manner and similarly inhibited the binding of a mutant defective in the major fimbrial subunits. It did not affect the binding of strains defective in FimD. These results prove that B. pertussis bind to human monocytes via the fimbrial minor subunit FimD.
引用
收藏
页码:924 / 929
页数:6
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