RESONANCE RAMAN STUDY OF THE DARK-ADAPTED FORM OF THE PURPLE MEMBRANE-PROTEIN

被引：28

作者：

ATON, B ^{[1
]}

DOUKAS, AG ^{[1
]}

CALLENDER, RH ^{[1
]}

BECHER, B ^{[1
]}

EBREY, TG ^{[1
]}

机构：

[1] UNIV ILLINOIS,DEPT PHYSIOL & BIOPHYS,URBANA,IL 61801

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 576卷 / 02期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

(Purple membrane); Bacteriorhodopsin; Chromaphore-protein linkage; Dark-adapted spectrum; Raman resonance;

D O I：

10.1016/0005-2795(79)90417-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The resonance Raman spectrum of the dark-adapted form of the purple membrane protein (bacteriorhodopsin) has been obtained and is compared to the light-adapted pigment and model chromophore spectra. As in the light-adapted form, the chromophore-protein linkage is found to be a protonated Schiff base. Electron delocalization appears to play the dominant role in color regulation. The dark-adapted spectrum indicates a conformation closer to 13-cis than the light-adapted spectrum. © 1979.

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收藏

页码：424 / 428

页数：5

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