DEMONSTRATION OF KININ-RELEASE IN THE PERITONEAL-EXUDATE OF KAOLIN-INDUCED WRITHING IN MICE

Using a bradykinin enzyme immunoassay, we measured the amount of kinin in the peritoneal washings of mice with the kaolin-induced writhing reaction. Simultaneous treatment with captopril, a kininase II inhibitor, significantly increased the kinin level at 1 min after kaolin injection. Soybean trypsin inhibitor injected simultaneously with kaolin almost completely suppressed the kinin level at 1 min with or without treatment of captopril. These results suggest that kinin is released through activation of the plasma kali i krei n-kin i n system by kaolin, and that kinin could be a main mediator for the writhing reaction. © 1990, The Japanese Pharmacological Society. All rights reserved.