PREPARATION OF PLANT-MATERIAL FOR ELEMENTAL ANALYSIS USING ESI AND EELS TECHNIQUES

被引：12

作者：

LEHMANN, H ^{[1
]}

KRAMER, A ^{[1
]}

SCHULZ, D ^{[1
]}

PROBST, W ^{[1
]}

机构：

[1] APPLIKAT LAB TEM,W-7082 OBERKOCHEN,GERMANY

来源：

ULTRAMICROSCOPY | 1990年 / 32卷 / 01期

关键词：

D O I：

10.1016/0304-3991(90)90090-9

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The optimal preparation of plant material is the prerequisite for elemental analysis using ESI or EELS techniques. Substances must not be extracted or shifted during the preparation procedure. In the present paper some examples are described for preparation techniques permitting elemental analysis. Silica can be analyzed in diatom valves and in Equisetum cell walls after conventional chemical fixation. Water-soluble calcium was precipitated as calcium antimonate or exchanged for insoluble lanthanum. The theoretically best method seems to be cryofixation, followed by freeze substitution or cryosectioning. © 1990.

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页码：26 / 34

页数：9

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