APPLICATION OF THE NEUTRAL RED ASSAY (NR ASSAY) TO MONOLAYER-CULTURES OF PRIMARY HEPATOCYTES - RAPID COLORIMETRIC VIABILITY DETERMINATION FOR THE UNSCHEDULED DNA-SYNTHESIS TEST (UDS)

被引：146

作者：

FAUTZ, R

HUSEIN, B

HECHENBERGER, C

机构：

[1] Cytotest Cell Research GmbH, Co. KG (CCR)

来源：

MUTATION RESEARCH | 1991年 / 253卷 / 02期

关键词：

NEUTRAL RED ASSAY; RAT HEPATOCYTES; CYTOTOXICITY; UNSCHEDULED DNA SYNTHESIS;

D O I：

10.1016/0165-1161(91)90130-Z

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The neutral red (NR) absorption method was adapted for the determination of cell viability in the UDS assay with primary hepatocyte cultures of the rat. The NR method is rapid, easy to perform, and suitable for handling of large numbers of cultures simultaneously. It can be used for concentration range-finding pre-experiments. In addition, it can easily be integrated into a UDS test protocol for documentation of toxic effects if supplementary cultures for each concentration are established. The time schedule required for the NR assay makes it possible for one person to process the hepatocytes for autoradiography and at the same time determine the toxicity.

引用

页码：173 / 179

页数：7

共 10 条

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