FURTHER EVALUATION OF A MODIFIED MICRONUCLEUS ASSAY WITH V79-CELLS FOR DETECTION OF ANEUGENIC EFFECTS

被引：20

作者：

SEELBACH, A

FISSLER, B

MADLE, S

机构：

[1] Max-von-Pettenkofer-Institute, Federal Health Office, D-14195 Berlin

来源：

MUTATION RESEARCH | 1993年 / 303卷 / 04期

关键词：

MICRONUCLEUS TEST WITH V79-CELLS; ANEUGENIC EFFECT;

D O I：

10.1016/0165-7992(93)90018-Q

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In an earlier publication, we reported on the development of a modified micronucleus assay with V79 cells enabling preferential detection of aneugen-induced micronuclei (Seelbach et al., 1993). Here we present a further evaluation of the modified micronucleus assay based on the investigation of seven further suspected aneugens. Five compounds gave positive results: cadmium chloride, chloral hydrate, hydroquinone, thimerosal and vinblastine. Econazole and pyrimethamine were negative. Up to now, our experience has shown that data produced by the modified V79/micronucleus assay are quite reliable: the variation of spontaneous micronucleus frequencies was low (0.8-1.7%) and the reproducibility of the data was good.

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页码：163 / 169

页数：7

相关论文

共 20 条

[1] INVITRO MICRONUCLEUS TEST WITH KINETOCHORE STAINING - EVALUATION OF TEST-PERFORMANCE [J].

ANTOCCIA, A ;

DEGRASSI, F ;

BATTISTONI, A ;

CILIUTTI, P ;

TANZARELLA, C .

MUTAGENESIS, 1991, 6 (04) :319-324

[2] MUTAGENICITY OF METHYL 2-BENZIMIDAZOLECARBAMATE, DIETHYLSTILBESTROL AND ESTRADIOL - STRUCTURAL CHROMOSOMAL-ABERRATIONS, SISTER-CHROMATID EXCHANGES, C-MITOSES, POLYPLOIDIES AND MICRONUCLEI [J].

BANDUHN, N ;

OBE, G .

MUTATION RESEARCH, 1985, 156 (03) :199-218

[3] MEMBRANE-DAMAGING AGENTS CAUSE MITOTIC NONDISJUNCTION IN ASPERGILLUS-NIDULANS [J].

BELLINCAMPI, D ;

GUALANDI, G ;

LAMONICA, E ;

POLEY, C ;

MORPURGO, GP .

MUTATION RESEARCH, 1980, 79 (02) :169-172

[4] THE ANALYSIS OF 10 POTENTIAL SPINDLE POISONS FOR THEIR ABILITY TO INDUCE CREST-POSITIVE MICRONUCLEI IN HUMAN-DIPLOID FIBROBLASTS [J].

BONATTI, S ;

CAVALIERI, Z ;

VIAGGI, S ;

ABBONDANDOLO, A .

MUTAGENESIS, 1992, 7 (02) :111-114

[5] PRODUCTION OF MICRONUCLEI FROM CHROMOSOME-ABERRATIONS IN IRRADIATED CULTURES OF HUMAN LYMPHOCYTES [J].

COUNTRYMAN, PI ;

HEDDLE, JA .

MUTATION RESEARCH, 1976, 41 (2-3) :321-331

[6] IMMUNOFLUORESCENT STAINING OF KINETOCHORES IN MICRONUCLEI - A NEW ASSAY FOR THE DETECTION OF ANEUPLOIDY [J].

DEGRASSI, F ;

TANZARELLA, C .

MUTATION RESEARCH, 1988, 203 (05) :339-345

[7] MEASUREMENT OF MICRONUCLEI IN LYMPHOCYTES [J].

FENECH, M ;

MORLEY, AA .

MUTATION RESEARCH, 1985, 147 (1-2) :29-36

[8] THE INDUCTION OF ANEUPLOIDY IN CULTURED CHINESE HAMSTER-CELLS BY PROPIONALDEHYDE AND CHLORAL HYDRATE [J].

FURNUS, CC ;

ULRICH, MA ;

TERREROS, MC ;

DULOUT, FN .

MUTAGENESIS, 1990, 5 (04) :323-326

[9] CHROMOSOME-ABERRATIONS AND SISTER CHROMATID EXCHANGES IN CHINESE-HAMSTER OVARY CELLS - EVALUATIONS OF 108 CHEMICALS [J].

GALLOWAY, SM ;

ARMSTRONG, MJ ;

REUBEN, C ;

COLMAN, S ;

BROWN, B ;

CANNON, C ;

BLOOM, AD ;

NAKAMURA, F ;

AHMED, M ;

DUK, S ;

RIMPO, J ;

MARGOLIN, BH ;

RESNICK, MA ;

ANDERSON, B ;

ZEIGER, E .

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 1987, 10 :1-175

[10] EFFECTS OF THE PRINCIPAL HYDROXY-METABOLITES OF BENZENE ON MICROTUBULE POLYMERIZATION [J].

IRONS, RD ;

NEPTUN, DA .

ARCHIVES OF TOXICOLOGY, 1980, 45 (04) :297-305