NEW QUANTITATIVE-DETERMINATION OF 2,3-DIOXOBUTANE (DIACETYL) IN ALCOHOLIC BEVERAGES BY AN INDIRECT SPECTROFLUOROMETRIC PROCEDURE WITH USE OF 2,3-DIAMINONAPHTALENE AS REAGENT OF DERIVATIZATION

The experimental conditions of an earlier spectrofluorimetric determination of 2,3-dioxobutane as 2,3-diaminonaphtalene derivative have been optimized. Fluorescence intensities are increased by a factor of 13 over the precedent ones. The calibration graphs are linear in the concentration range 2.5-130 mumol/l in spectrophotometry and 1.75 x 10(-1)-2.5 mumol/l in fluorimetry. Limits of detection are very small: 7 x 10(-2) and 2.5 x 10(-2) mumol/l, respectively. Applications to alcoholic beverages are described. The new fluorimetric technique is simple and rapid since it does not require steam distillation.