MECHANISMS UNDERLYING ANTINOCICEPTION PROVOKED BY HETEROSEGMENTAL NOXIOUS-STIMULATION IN THE RAT TAIL-FLICK TEST

被引:34
作者
PITCHER, GM
YASHPAL, K
CODERRE, TJ
HENRY, JL
机构
[1] MCGILL UNIV, DEPT PHYSIOL, MONTREAL, PQ H3G 1Y6, CANADA
[2] MCGILL UNIV, DEPT PSYCHIAT, MONTREAL, PQ H3G 1Y6, CANADA
[3] CLIN RES INST MONTREAL, PAIN MECH LAB, MONTREAL, PQ H3G 1Y6, CANADA
基金
加拿大健康研究院;
关键词
D O I
10.1016/0306-4522(94)00477-M
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Physiological studies were conducted to examine the effects of noxious stimulation of one hindpaw or one forepaw on the latency of the withdrawal reflex in the tail-flick test in lightly anesthetized spinally intact or transected rats. Male Sprague-Dawley rats were anesthetized with an intraperitoneal injection of a mixture of Na-pentobarbital (20 mg/kg) and chloral hydrate (120 mg/kg). After baseline readings were taken in the tail-flick test, the effects of various noxious stimuli applied to remote body regions were assessed. The noxious stimuli included unilateral or bilateral hindpaw or unilateral forepaw thermal (immersion in water at 55 degrees C for 90 s), unilateral or bilateral chemical (subcutaneous hindpaw injection of 50 mu l of 5% formalin) and unilateral or bilateral mechanical (pinch with clamp exerting a force of 14.75 or 27 N) stimulation. Bilateral chemical and thermal, and unilateral thermal stimulation induced an antinociceptive response, consisting of an increase in tail-flick latency, peaking at 30 s after stimulation. Recovery to baseline levels occurred over the next 3-6 min. The antinociceptive effect of noxious thermal stimulation was attenuated or absent in chronically spinalized animals (T6/7) following hindpaw or forepaw immersion, respectively. Noxious mechanical stimulation had no effect on tail-flick latency. The data provide evidence that a noxious thermal or chemical stimulus produces a heterosegmental antinociceptive effect which is mediated in part via a supraspinal mechanism and in part via a local spinal mechanism. As an effect of noxious thermal stimulation was seen in spinally transected rats and as noxious mechanical stimulatiion failed to evoke an effect in spinally intact rats, the data indicate that the antinociceptive responses in this paradigm are different from those associated with DNIC (diffuse noxious inhibitory controls).
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页码:273 / 281
页数:9
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