IMMUNOHISTOLOGICAL DEMONSTRATION OF FELINE INFECTIOUS PERITONITIS VIRUS-ANTIGEN IN PARAFFIN-EMBEDDED TISSUES USING FELINE ASCITES OR MURINE MONOCLONAL-ANTIBODIES

被引：50

作者：

TAMMER, R

EVENSEN, O

LUTZ, H

REINACHER, M

机构：

[1] UNIV LEIPZIG,INST VET PATHOL,D-04103 LEIPZIG,GERMANY

[2] UNIV GIESSEN,INST VET PATHOL,D-35392 GIESSEN,GERMANY

[3] NATL VET INST,N-0033 OSLO,NORWAY

[4] UNIV ZURICH,VET MED LABOR,CH-8057 ZURICH,SWITZERLAND

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1995年 / 49卷 / 1-2期

关键词：

CAT DISEASE; FELINE INFECTIOUS PERITONITIS (FIP); FELINE CORONAVIRUS; MONOCLONAL ANTIBODIES;

D O I：

10.1016/0165-2427(95)05459-J

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Feline infectious peritonitis (FIP) virus antigen was demonstrated after methanol, ethanol or formalin fixation in paraffin-embedded tissues by means of monoclonal and polyclonal antibodies. The monoclonal antibody was induced by immunization with transmissible gastroenteritis virus. Polyclonal antibodies were obtained by purification on protein A-Sepharose of ascites fluid from a cat with FIP. Almost all cats diagnosed as suffering from FIP by postmortem and histological examination exhibited FIP virus (FIPV) antigen in macrophages in granulomas whereas FIPV antigen was only once demonstrable in another location.

引用

页码：177 / 182

页数：6

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