DIRECTED TRANSPOSITION OF ARABINOSE OPERON - A TECHNIQUE FOR ISOLATION OF SPECIALIZED TRANSDUCING BACTERIOPHAGES FOR ANY ESCHERICHIA COLI GENE

An F-thr-ara‡ ‡ Abbreviations used: lac, ara, gal, utilization of lactose, arabinose, galactose; his, pyr, pur, arg, thr, leu, pro, trp requirement for histidine, pyrimidine, purine, arginine, threonine, leucine, proline, tryptophan; T1r, T1,5r, resistance to phage T1, phages T1 and T5; att80, chromosome attachment site for φ80; Sms, Smr, sensitive and resistant to streptomycin; φ80dara, φ80dlac, defective φ80 transducing phage carrying the ara region, the lac region; FTS, F factor with mutation rendering its replication temperature sensitive; φ80h, φ80 host range; φ80v, φ80 virulent; λ hφ80, λ-φ80 hybrid having λ immunity and φ80 host range and attachment specificity. episome has been inserted into the Escherichia coli chromosome at the T1 locus near the attachment site for bacteriophage φ80. From a strain carrying such an insertion, a φ80dara transducing phage was isolated. The techniques used should be applicable for the isolation of specialized transducing phages for many E. coli genes. © 1969.