PLASMODIUM-FALCIPARUM - GENETIC STABILITY OF THE UGANDA PALO-ALTO STRAIN PROPAGATED IN THE SQUIRREL-MONKEY (SAIMIRI-SCIUREUS)

被引：13

作者：

FANDEUR, T ^{[1
]}

MERCEREAUPUIJALON, O ^{[1
]}

机构：

[1] INST PASTEUR,DEPT IMMUNOL,UNITE PARASITOL EXPTL,F-75724 PARIS 15,FRANCE

来源：

EXPERIMENTAL PARASITOLOGY | 1991年 / 72卷 / 03期

关键词：

PLASMODIUM-FALCIPARUM; GENETIC STABILITY; SAIMIRI-SCIUREUS; UGANDA PALO-ALTO STRAIN; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SOUTHERN BLOT; POLYMERASE CHAIN REACTION (PCR); ETHYLENEDIAMINETETRAACETIC ACID (EDTA); DEOXYRIBONUCLEIC ACID (DNA);

D O I：

10.1016/0014-4894(91)90141-I

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The Uganda Palo Alto strain of Plasmodium falciparum has been extensively used in several laboratories to infect Saimiri monkeys. In the study reported here three distinct lines derived by parallel serial blood transfers from a single 10-year-old passage of the Palo Alto strain were examined for genetic diversity using the polymerase chain reaction and restriction mapping techniques. A comparison of the PF 11.1, P190, MSA2, S-Ag, and KAHRP genes indicates that these parasite lines are apparently homogeneous and stable. Nevertheless, microheterogeneity was observed with molecular probes which are known to easily detect restriction fragment length polymorphisms (rep 20 and telomeric probes). These data show that the genetic characters of the strain can be considered monomorphic and are conserved after multiple passages in the squirrel monkey. © 1991.

引用

页码：223 / 235

页数：13

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