NONRADIOACTIVE LABELING OF RNA TRANSCRIPTS INVITRO WITH THE HAPTEN DIGOXIGENIN (DIG) - HYBRIDIZATION AND ELISA-BASED DETECTION

We have developed a system for the enzymatic in vitro synthesis of non-radioactively labeled RNA which is derivatized with the hapten digoxigenin (DIG). The labeling reaction as well as the conditions for hybridization and detection of hybrids by an antibody-conjugate and a coupled colour reaction were analyzed and adapted for high sensitivity and low background. In addition, data on the performance and sensitivity of digoxigenin-labeled RNA probes in Southern and Northern blots are presented. © 1990 Oxford University Press.