THE PREFERENTIAL UPTAKE OF VERY-LOW-DENSITY LIPOPROTEIN CHOLESTERYL ESTER BY RAT-LIVER INVIVO

被引:10
作者
HOLDER, JC
ZAMMIT, VA
ROBINSON, DS
机构
[1] UNIV LEEDS,DEPT BIOCHEM,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND
[2] HANNAH RES INST,AYR KA6 5HL,SCOTLAND
关键词
D O I
10.1042/bj2720735
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The removal from the blood and the uptake by the liver of injected very-low-density lipoprotein (VLDL) preparations that had been radiolabelled in their apoprotein and cholesteryl ester moieties was studied in lactating rats. Radiolabelled cholesteryl ester was removed from the blood and taken up by the liver more rapidly than sucrose-radiolabelled apoprotein. Near-maximum cholesteryl ester uptake by the liver occurred within 5 min of the injection of the VLDL. At this time, apoprotein B uptake by the liver was only about 25% of the maximum. Maximum uptake of the injected VLDL apoprotein B label was not achieved until at least 15 min after injection, by which time the total uptakes of cholesteryl ester and apoprotein B label were very similar. The results suggest that preferential uptake of the lipoprotein cholesteryl ester by the liver occurred before endocytosis of the entire lipoprotein complex. The fate of the injected VLDL cholesteryl ester after its uptake by the liver was also monitored. Radiolabel associated with the hepatic cholesteryl ester fraction fell steadily from its early maximum level, the rate of fall being faster and more extensive when the fatty acid, rather than the cholesterol, moiety of the ester was labelled. By 30 min after the injection of VLDL containing [H-3]cholesteryl ester, over one-third of the injected label was already present as [H-3]cholesterol in the liver. When VLDL containing cholesteryl [C-14]oleate was injected, a substantial proportion (about 25%) of the injected radiolabelled fatty acid appeared in the hepatic triacylglcerol fraction within 60 min: very little was present in the plasma triacylglycerol fraction at this time.
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页码:735 / 741
页数:7
相关论文
共 40 条
[1]   THE UPTAKE OF THE APOPROTEIN AND CHOLESTERYL ESTER OF HIGH-DENSITY-LIPOPROTEINS BY THE PERFUSED-RAT-LIVER [J].
ARBEENY, CM ;
RIFICI, VA ;
EDER, HA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 917 (01) :9-17
[2]  
BARTLETT GR, 1959, J BIOL CHEM, V234, P466
[3]  
BUCOLO G, 1973, CLIN CHEM, V19, P476
[4]   UPTAKE OF CHYLOMICRON [CHOLESTERYL-H-3 LINOLEYL ETHER BY MESENCHYMAL RAT-HEART CELL-CULTURES [J].
CHAJEKSHAUL, T ;
FRIEDMAN, G ;
HALPERIN, G ;
STEIN, O ;
STEIN, Y .
BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 666 (01) :147-155
[5]   ROLE OF LIPOPROTEIN-LIPASE IN THE UPTAKE OF CHOLESTERYL ESTER BY RAT LACTATING MAMMARY-GLAND INVIVO [J].
CHAJEKSHAUL, T ;
FRIEDMAN, G ;
HALPERIN, G ;
STEIN, O ;
STEIN, Y .
BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 666 (02) :216-222
[6]  
CLARK RW, 1986, CANCER RES, V46, P1894
[7]   LIPOPROTEIN METABOLISM AND THE ROLE OF APOLIPOPROTEINS AS METABOLIC PROGRAMMERS [J].
DOLPHIN, PJ .
CANADIAN JOURNAL OF BIOCHEMISTRY AND CELL BIOLOGY, 1985, 63 (08) :850-869
[8]  
EGUSA G, 1983, J LIPID RES, V24, P1261
[9]   METABOLISM OF APOPROTEIN-B OF PLASMA VERY LOW-DENSITY LIPOPROTEINS IN RAT [J].
FAERGEMAN, O ;
SATA, T ;
KANE, JP ;
HAVEL, RJ .
JOURNAL OF CLINICAL INVESTIGATION, 1975, 56 (06) :1396-1403
[10]   METABOLISM OF CHOLESTERYL ESTERS OF RAT VERY LOW-DENSITY LIPOPROTEINS [J].
FAERGEMAN, O ;
HAVEL, RJ .
JOURNAL OF CLINICAL INVESTIGATION, 1975, 55 (06) :1210-1218