INVITRO REGENERATION AND MICROPROPAGATION OF ALOE-VERA L

Rapid propagation by the formation of shoots from calli of Aloe vera was obtained in the present investigation. Callus formation was induced in stem segments from young axillary shoots grown on the underground rhizomatous stem. The use of polyvinylpyrrolidone (PVP) in the nutrient media reduced the secretion of phenolic substances from the explant. Murashige and Skoog's basal medium containing 1 mg l-1 2,4-dichlorophenoxyacetic acid and 0.2 mg l-1 kinetin gave the best callus induction. Shoots were initiated from the calli with reduced 2,4-D and increased kinetin concentration.