PURIFICATION AND CHARACTERIZATION OF A COFFEA-CANEPHORA ALPHA-D-GALACTOSIDASE ISOZYME

被引：23

作者：

HAIBACH, F ^{[1
]}

HATA, J ^{[1
]}

MITRA, M ^{[1
]}

DHAR, M ^{[1
]}

HARMATA, M ^{[1
]}

SUN, P ^{[1
]}

SMITH, D ^{[1
]}

机构：

[1] UNIV MISSOURI,DEPT CHEM,COLUMBIA,MO 65201

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 181卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)92117-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Exoglycosidases modify carbohydrate epitopes on glycoproteins and glycolipids. The α-D-galactosidase from Coffea canephora is an important exoglycosidase which degrades the human blood group B epitope. Although multiple isozymes have been described, they have never been demonstrably purified and thoroughly characterized. We have developed a technique to purify an isozyme to homogeneity. The isolated enzyme has a molecular weight of 36.7 kDa by SDS PAGE and 34.0 kDa by gel filtration. The isozyme is highly selective for α-D-galactosides and inactive against other low molecular weight substrates. It hydrolyzes the the terminal α-D-galactosyl residue from the blood group B epitope. Protease activity is below detectable limits. The isozyme has a broad pH optima at 6.3, a pl of 7.03, is unaffected by ionic strength, and is stable at 4° C. © 1991.

引用

页码：1564 / 1571

页数：8

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