CLONING AND CHARACTERIZATION OF 2 NEW CDNAS ENCODING MURINE TRIPLE LIM DOMAINS

被引：13

作者：

DIVECHA, N

CHARLESTON, B

机构：

[1] INST ANIM HLTH,CONTRACTS GRP,NEWBURY RG16 0NN,BERKS,ENGLAND

[2] BABRAHAM INST,CAMBRIDGE CB2 4AT,ENGLAND

[3] UNIV LONDON ROYAL VET COLL,HATFIELD AL9 7TA,HERTS,ENGLAND

来源：

GENE | 1995年 / 156卷 / 02期

基金：

英国惠康基金;

关键词：

RECOMBINANT DNA; ZYXIN; ZINC FINGER; PHAGE LAMBDA-ZAP CDNA LIBRARY; NUCLEOTIDE SEQUENCE; MOUSE; TESTIS;

D O I：

10.1016/0378-1119(95)00088-N

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have cloned and sequenced two new cDNAs that code for proteins carrying the related triple LIM domains (acronym of Lin-11, Isl-1, Mec-3) proteins. These LIM domains show good agreement to the LIM domain consensus sequence, but also exhibit some novel variations, The 1.36-and 2.8-kb cDNAs are probably splice variants of one gene and code for 42- and 50-kDa proteins, respectively. The larger transcript has a 900-nucleotide (nt) 3' untranslated region (UTR), High levels of the 2.8-kb transcript can be detected in many tissues, and all tissues show some level of expression of both transcripts, the larger transcript being more abundant. In adult testis there are very high levels of the 1.36-kb transcript and moderate levels of the 2.8-kb transcript. The wide tissue distribution and high levels of expression suggest an important role for these proteins in cellular function.

引用

页码：283 / 286

页数：4

共 16 条

[1] Bird, CpG-rich islands and the function of methylation, Nature, 321, pp. 209-213, (1986)
[2] Birkenmeier, Gordon, Developmental regulation of a gene that encodes a cysteine-rich intestinal protein and maps near the murine immunoglobulin heavy chain locus, Proc. Natl. Acad. Sci. USA, 83, pp. 2516-2520, (1986)
[3] Brooksbank, Hutchings, Butcher, Irvine, Divecha, Monoclonal antibodies to phosphatidylinositol 4-phosphate 5-kinase: distribution and intracellular localization of the C isoform, Biochem. J., 291, pp. 77-82, (1993)
[4] Chomczynski, Sacchi, Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction, Anal. Biochem., 162, pp. 156-159, (1989)
[5] Crawford, Pino, Beckerle, Biochemical and molecular characterization of the chicken cysteine-rich protein, a developmentally regulated LIM-domain protein that is associated with the actin cytoskeleton, J. Cell Biol., 124, pp. 117-127, (1994)
[6] Devereux, Haeberli, Smithies, A comprehensive set of sequence analysis programs for the VAX, Nucleic Acids Res., 12, pp. 387-395, (1984)
[7] Freyd, Kim, Horvitz, Novel cysteine-rich motif and homeodomain in the product of the Caenorhabitis elegans cell lineage gene lin-11, Nature, 344, pp. 876-879, (1990)
[8] Karlsson, Thor, Norbert, Ohlsson, Edlund, Insulin gene enhancer binding protein Isl-1 is a member of a novel class of proteins containing both a homeo- and a Cys-His domain, Nature, 344, pp. 879-882, (1990)
[9] Klug, Co-chairman's remarks: protein designs for specific recognition of DNA, Gene, 135, pp. 83-92, (1993)
[10] Nalik, Panayotova-Heiermann, Pongs, Characterization of an estradiol-stimulated mRNA in the brain of adult male rats, Mol. Cell. Endocrinol., 62, pp. 235-242, (1989)

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