PRIMARY STRUCTURE AND PARTIAL CHARACTERIZATION OF A LIFE-CYCLE-REGULATED CYSTEINE PROTEASE FROM TRYPANOSOMA (NANNOMONAS) CONGOLENSE

被引：20

作者：

FISH, WR ^{[1
]}

NKHUNGULU, ZM ^{[1
]}

MURIUKI, CW ^{[1
]}

NDEGWA, DM ^{[1
]}

LONSDALEECCLES, JD ^{[1
]}

STEYAERT, J ^{[1
]}

机构：

[1] INT LAB RES ANIM DIS,NAIROBI,KENYA

来源：

GENE | 1995年 / 161卷 / 01期

关键词：

AFRICAN TRYPANOSOME; PATHOGEN; PARASITE; CATHEPSIN; PAPAIN; PROTEINASE; ANTIGEN; MESSENGER-RNA; POLYPYRIMIDINE;

D O I：

10.1016/0378-1119(95)00304-O

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Trypanosoma (Nannomonas) congolense is an important pathogenic parasite of domestic livestock in Africa. We have cloned a cDNA encoding a prepro-cysteine protease of this protozoan, the sequence of which indicates it is an early mRNA processing intermediate. Northern analysis demonstrates a life-cycle-stage specificity similar to previously described enzymatic data. The deduced amino-acid sequence shows extensive similarity to cysteine proteases of other parasitic protozoa, as well as papain and cathepsin L. As with other African trypanosomes, a poly-proline tract connects the catalytic domain with an unusual C-terminal extension,

引用

页码：125 / 128

页数：4

共 24 条

[1] THE C-TERMINAL EXTENSION OF THE MAJOR CYSTEINE PROTEINASE (CRUZIPAIN) FROM TRYPANOSOMA-CRUZI [J].