DETERMINATION OF CYANIDE USING A TYROSINASE AMPEROMETRIC BIOSENSOR WITH CATECHOL AS SUBSTRATE

被引：26

作者：

HU, XY

LENG, ZZ

机构：

来源：

ANALYST | 1995年 / 120卷 / 05期

关键词：

CYANIDE DETERMINATION; AMPEROMETRY; BIOSENSOR; ENZYME ELECTRODES; ENZYME INHIBITION;

D O I：

10.1039/an9952001555

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Low cyanide concentrations are determined with a tyrosinase amperometric biosensor using enzyme immobilized on a preactivated polyamide membrane that is closely attached to the end of a wax-impregnated graphite electrode. The test is performed in the presence of the catechol substrate at -0.200 V versus SCE in 0.1 mol l(-1) phosphate buffer (pH 7.5) (the reduction current results from o-benzoquinone, derived from the tyrosinase driving the oxidation of catechol, which is being measured). The decrease in substrate response within the linear range 2.0 X 10(-7)-4.0 x 10(-5) mol l(-1) is proportional to the square-root of cyanide concentration. This method is characterized by high selectivity and reproducibility.

引用

页码：1555 / 1557

页数：3

共 13 条

[1] THE REACTION OF CN- WITH THE BINUCLEAR COPPER SITE OF NEUROSPORA TYROSINASE - ITS RELEVANCE FOR A COMPARISON BETWEEN TYROSINASE AND HEMOCYANIN ACTIVE-SITES [J].