ADENOPHOSTIN-MEDICATED QUANTAL CA2+ RELEASE IN THE PURIFIED AND RECONSTITUTED INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR-TYPE-1

被引：78

作者：

HIROTA, J

MICHIKAWA, T

MIYAWAKI, A

TAKAHASHI, M

TANZAWA, K

OKURA, I

FURUICHI, T

MIKOSHIBA, K

机构：

[1] TOKYO INST TECHNOL, DEPT BIOENGN, MIDORI KU, YOKOHAMA, KANAGAWA 226, JAPAN

[2] SANKYO CO LTD, BIOL RES LABS, TOKYO 140, JAPAN

[3] INST PHYS & CHEM RES, TSUKUBA LIFE SCI CTR, MOLEC NEUROBIOL LAB, TSUKUBA, IBARAKI 305, JAPAN

来源：

FEBS LETTERS | 1995年 / 368卷 / 02期

关键词：

INOSITOL 1,4,5-TRISPHOSPHATE; INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR; QUANTAL CA2+ RELEASE; ADENOPHOSTIN;

D O I：

10.1016/0014-5793(95)00659-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Kinetics of Ca2+ release by adenophostin, a novel agonist of inositol 1,4,5-trisphosphate (IP3) receptor, in the purified and reconstituted IP3 receptor type 1 (IP(3)R1) was investigated using the fluorescent Ca2+ indicator fluo-3. Submaximal concentrations of adenophostin caused quantal Ca2+ release from the purified IP(3)R1 as IP3 did. Adenophostin-induced Ca2+ release by the purified IP(3)R1 exhibited a high positive cooperativity (nH = 3.9 +/- 0.2, EC(50) = 11 nM), whereas the IP3-induced Ca2+ release exhibited a moderate one (nH = 1.8 +/- 0.1, EC(50) = 100 nM), Inhibition of [H-3]IP3 binding to the purified IP(3)R1 by adenophostin exhibited a positive cooperativity (nH = 1.9, K-i = 10 nM), whereas IP3 did not (nH = 1.1, K-i = 41 nM).

引用

页码：248 / 252

页数：5

共 18 条

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