CLONING AND CHARACTERIZATION OF THE HUMAN V3 PITUITARY VASOPRESSIN RECEPTOR

被引：156

作者：

DEKEYZER, Y

AUZAN, C

LENNE, F

BELDJORD, C

THIBONNIER, M

BERTAGNA, X

CLAUSER, E

机构：

[1] UNIV PARIS 05,INST COCHIN GENET MOLEC,INSERM,U129,PARIS,FRANCE

[2] COLL FRANCE,INSERM,U36,F-75231 PARIS,FRANCE

[3] CASE WESTERN RESERVE UNIV,SCH MED,DEPT MED,CLEVELAND,OH 44106

来源：

FEBS LETTERS | 1994年 / 356卷 / 2-3期

关键词：

ARGININE-VASOPRESSIN; PITUITARY; PHOSPHOLIPASE C; G-PROTEIN COUPLED RECEPTOR; ACTH;

D O I：

10.1016/0014-5793(94)01268-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Arginine-vasopressin (AVP) prays a determinant role in the normal ACTH response to stress in mammals. We cloned a human cDNA coding a 424 amino acid G-protein coupled receptor structurally related to the vasopressin/oxytocin receptor family. When expressed in COS cells, this receptor binds AVP with a high affinity (K-d = 0.55 +/- 0.13 nM) and is functionally coupled to phospholipase C. Competition studies with peptidic or non peptidic AVP analogues reveal that it is pharmacologically distinct from V1a and V2 AVP receptors and therefore it is designated V3. RT-PCR analysis shows that the human V3 receptor is expressed in normal pituitary and also in kidney, but is undetectable in liver, myometrium and adrenal gland. Northern blot analysis reveals a similar to 4.8 kb messenger in human corticotropic pituitary adenomas.

引用

页码：215 / 220

页数：6

共 25 条

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