A GENE-CLUSTER INVOLVED IN THE UTILIZATION OF BOTH FREE HEME AND HEME-HEMOPEXIN BY HAEMOPHILUS-INFLUENZAE TYPE-B

被引:117
作者
COPE, LD
YOGEV, R
MULLEREBERHARD, U
HANSEN, EJ
机构
[1] UNIV TEXAS, SW MED CTR, DEPT MICROBIOL, DALLAS, TX 75235 USA
[2] CHILDRENS MEM HOSP, DIV INFECT DIS, CHICAGO, IL 60614 USA
[3] CORNELL UNIV, SCH MED, DEPT PEDIAT, NEW YORK, NY 10021 USA
[4] CORNELL UNIV, SCH MED, DEPT BIOCHEM, NEW YORK, NY 10021 USA
[5] CORNELL UNIV, SCH MED, DEPT PHARMACOL, NEW YORK, NY 10021 USA
关键词
D O I
10.1128/jb.177.10.2644-2653.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The utilization of heme bound to the serum glycoprotein hemopexin by Haemophilus influenzae type b (Hib) strain DL42 requires the presence of the 100-kDa heme:hemopexin-binding protein encoded by the hxuA gene (M. S. Hanson, S. E. Pelzel, J. Latimer, U. Muller-Eberhard, and E. J. Hansen, Proc. Natl. Acad. Sci. USA 89:1973-1977, 1992). Nucleotide sequence analysis of a 5-kb region immediately upstream from the hxuA gene revealed the presence of two genes, designated hxuC and hxuB, which encoded outer membrane proteins. The 78-kDa HxuC protein had similarity to TonB-dependent outer membrane proteins of other organisms, whereas the 60-kDa HxuB molecule most closely resembled the ShlB protein of Serratia marcescens. A set of three isogenic Hib mutants with cat cartridges inserted individually into their hxuA, hxuB, and hxuC genes was constructed. None of these mutants could utilize heme:hemopexin. The hxuC mutant was also unable to utilize low levels of free heme, whereas both the hxuA and hxuB mutants could utilize free heme. When the wild type hxuC gene was present in trans, the hxuC mutant regained its ability to utilize low levels of free heme but still could not utilize heme:hemopexin. The hxuA mutant could utilize heme:hemopexin when a functional hxuA gene from a nontypeable H. influenzae strain was present in trans. Complementation analysis using this cloned nontypeable H. influenzae hxuA gene also indicated that the HxuB protein likely functions in the release of soluble HxuA from the Hib cell. These studies indicate that at least two and possibly three gene products are required for utilization of heme bound to hemopexin by Hib strain DL42.
引用
收藏
页码:2644 / 2653
页数:10
相关论文
共 60 条
  • [1] ALEXANDER HE, 1965, BACTERIAL MYCOTIC IN, P724
  • [2] BARCAK GJ, 1991, METHOD ENZYMOL, V204, P321
  • [3] GENETIC SUPPRESSION DEMONSTRATES INTERACTION OF TONB PROTEIN WITH OUTER-MEMBRANE TRANSPORT PROTEINS IN ESCHERICHIA-COLI
    BELL, PE
    NAU, CD
    BROWN, JT
    KONISKY, J
    KADNER, RJ
    [J]. JOURNAL OF BACTERIOLOGY, 1990, 172 (07) : 3826 - 3829
  • [4] SERRATIA-MARCESCENS FORMS A NEW TYPE OF CYTOLYSIN
    BRAUN, V
    HOBBIE, S
    ONDRACZEK, R
    [J]. FEMS MICROBIOLOGY LETTERS, 1992, 100 (1-3) : 299 - 305
  • [5] M13 BACTERIOPHAGE AND PUC PLASMIDS CONTAINING DNA INSERTS BUT STILL CAPABLE OF BETA-GALACTOSIDASE ALPHA-COMPLEMENTATION
    CLOSE, TJ
    CHRISTMANN, JL
    RODRIGUEZ, RL
    [J]. GENE, 1983, 23 (02) : 131 - 136
  • [6] THE 100 KDA HEME-HEMOPEXIN-BINDING PROTEIN OF HAEMOPHILUS-INFLUENZAE - STRUCTURE AND LOCALIZATION
    COPE, LD
    THOMAS, SE
    LATIMER, JT
    SLAUGHTER, CA
    MULLEREBERHARD, U
    HANSEN, EJ
    [J]. MOLECULAR MICROBIOLOGY, 1994, 13 (05) : 863 - 873
  • [7] EFFECT OF MUTATIONS IN LIPOOLIGOSACCHARIDE BIOSYNTHESIS GENES ON VIRULENCE OF HAEMOPHILUS-INFLUENZAE TYPE-B
    COPE, LD
    YOGEV, R
    MERTSOLA, J
    ARGYLE, JC
    MCCRACKEN, GH
    HANSEN, EJ
    [J]. INFECTION AND IMMUNITY, 1990, 58 (07) : 2343 - 2351
  • [8] MOLECULAR-CLONING OF A GENE INVOLVED IN LIPOOLIGOSACCHARIDE BIOSYNTHESIS AND VIRULENCE EXPRESSION BY HAEMOPHILUS-INFLUENZAE TYPE-B
    COPE, LD
    YOGEV, R
    MERTSOLA, J
    LATIMER, JL
    HANSON, MS
    MCCRACKEN, GH
    HANSEN, EJ
    [J]. MOLECULAR MICROBIOLOGY, 1991, 5 (05) : 1113 - 1124
  • [9] CHARACTERIZATION OF A MUTANT OF HAEMOPHILUS-INFLUENZAE TYPE-B LACKING THE P2 MAJOR OUTER-MEMBRANE PROTEIN
    COPE, LD
    PELZEL, SE
    LATIMER, JL
    HANSEN, EJ
    [J]. INFECTION AND IMMUNITY, 1990, 58 (10) : 3312 - 3318
  • [10] IRON UPTAKE IN PLESIOMONAS-SHIGELLOIDES - CLONING OF THE GENES FOR THE HEME-IRON UPTAKE SYSTEM
    DASKALEROS, PA
    STOEBNER, JA
    PAYNE, SM
    [J]. INFECTION AND IMMUNITY, 1991, 59 (08) : 2706 - 2711