ANTIGENIC STRUCTURE OF CHIMERAS OF TYPE-1 AND TYPE-3 POLIOVIRUS INVOLVING ANTIGENIC SITE-1

被引：25

作者：

MINOR, PD ^{[1
]}

FERGUSON, M ^{[1
]}

KATRAK, K ^{[1
]}

WOOD, D ^{[1
]}

JOHN, A ^{[1
]}

HOWLETT, J ^{[1
]}

DUNN, G ^{[1
]}

BURKE, K ^{[1
]}

ALMOND, JW ^{[1
]}

机构：

[1] UNIV READING,DEPT MICROBIOL,READING RG1 5AQ,BERKS,ENGLAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1990年 / 71卷

关键词：

D O I：

10.1099/0022-1317-71-11-2543

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chimeric polioviruses have been prepared in which part of the antigenic site 1-encoding sequence of the Sabin strain of type 1 poliovirus has been replaced by sequences based on those found in the homologous region of the Sabin type 3 strain. The chimeras were analysed for their reaction with polyclonal and monoclonal antibodies raised against type 1 and type 3 viruses, and with polyclonal antipeptide sera, as well as for their immunogenicity in animals. The effectiveness with which the type 3 site was presented antigenically varied in ways which were partially predictable, based on the behaviour of type 3 mutants with monoclonal antibodies. However, other factors were implicated which may include conformational effects and other components of the site in addition to those altered in the chimeras. The ability of the chimeras to induce antibodies reacting with type 3 polioviruses paralleled their antigenic reactivity, and evidence is presented for the induction of strain-specific antibodies.

引用

页码：2543 / 2551

页数：9

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