EPR CHARACTERIZATION OF MOLECULAR TARGETS FOR NO IN MAMMALIAN-CELLS AND ORGANELLES

被引：340

作者：

HENRY, Y

LEPOIVRE, M

DRAPIER, JC

DUCROCQ, C

BOUCHER, JL

GUISSANI, A

机构：

[1] INST BIOCHIM, CNRS, URA 1116, F-91405 ORSAY, FRANCE

[2] INST CURIE, INSERM, U196, F-75005 PARIS, FRANCE

[3] INST CHIM SUBST NAT, CNRS, F-91198 GIF SUR YVETTE, FRANCE

[4] UNIV PARIS 05, CNRS, URA 400, F-75270 PARIS, FRANCE

来源：

FASEB JOURNAL | 1993年 / 7卷 / 12期

关键词：

NITRIC OXIDE; ELECTRON PARAMAGNETIC RESONANCE; METALLOPROTEINS;

D O I：

10.1096/fasebj.7.12.8397130

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nitric oxide is synthesized in mammalian cells from L-arginine or from pharmaceutical drugs. It forms paramagnetic complexes with some metalloproteins, inhibiting key enzymes in DNA synthesis, mitochondrial respiration, iron metabolism, etc. This article reviews how electron paramagnetic resonance spectroscopy helps to detect unambiguously such specific molecular targets for NO in mammalian whole cells and organelles. EPR has also been used for the detection of spin adducts of free NO by spin-trapping methods.

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页码：1124 / 1134

页数：11

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