SPECTROPHOTOMETRIC ASSAY OF A PENEM (SCH-29482) BY REACTION WITH IMIDAZOLE

A rapid method is described for the determination of SCH 29482. This is based on the spectrophotometric measurement at 386 nm of the product of reaction with imidazole formed at 35°C in a 1.5 M imidazole and 2 x 10-3M mercuric chloride solution at pH 7.50. The method, which permits detection of concentrations of SCH 29482 as low as to 2.8 ^g/ml, is reproducible and specific for intact SCH 29482 in the presence of degradation products. The molar absorptivity for the chromophore formed is 2.54 x 1041 mol'1 cm-1. © 1990, Taylor & Francis Group, LLC. All rights reserved.