FLUOROMETRIC-DETERMINATION OF TRYPTOPHAN IN HUMAN SERUM USING PHENYLGLYOXAL

被引：9

作者：

KOJIMA, E

KAI, M

OHKURA, Y

机构：

[1] Faculty of Pharmaceutical Sciences, Kyushu University 62, Fukuoka 812, Maidashi

来源：

ANALYTICAL SCIENCES | 1993年 / 9卷 / 01期

关键词：

TRYPTOPHAN; PHENYLGLYOXAL; FLUOROMETRY; SERUM;

D O I：

10.2116/analsci.9.25

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple fluorometric method is described for the determination of the total (free + albumin-bound) and free tryptophan in human serum. After deproteinization of the serum (perchloric acid precipitation for total tryptophan; ultrafiltration for free tryptophan), the samples are treated with phenylglyoxal in dilute perchloric acid to develop fluorescence. The method is sensitive and fairly selective for tryptophan in serum; the limit of detection is 0.84 nmol ml-1 in serum, thus giving a fluorescence intensity that is twice that of the blank.

引用

页码：25 / 27

页数：3

共 23 条

[1] DETERMINATION OF TYROSINE AND TRYPTOPHAN IN PROTEINS [J].