RAPID FLUOROMETRIC MEASUREMENT OF THE INTRACELLULAR CONCENTRATION OF CIPROFLOXACIN IN MOUSE PERITONEAL-MACROPHAGES

被引：9

作者：

CHATEAU, MT ^{[1
]}

CARAVANO, R ^{[1
]}

机构：

[1] UNIV MONTPELLIER 1,FAC PHARM,F-34060 MONTPELLIER,FRANCE

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 1993年 / 31卷 / 02期

关键词：

D O I：

10.1093/jac/31.2.281

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A simple fluorometric assay requiring only a single sample of cells to determine the number of cells, from the DNA linked to the fluorochrome 4′,6-diamidino-2- phenylindol (DAPI), and the uptake of ciprofloxacin, a natural fluorescent quinolone is described. Mouse peritoneal macrophages were found to concentrate ciprofloxacin up to 12·7 (± 1·5)-fold. Combined fluorometry provides a precise, sensitive method for determining the intracellular concentration of fluoroquinolones, as well as that of naturally fluorescent or fluorochrome-linked drugs. © 1993 The British Society for Antimicrobial Chemotherapy.

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页码：281 / 287

页数：7

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