PREFERENTIAL BINDING OF CROSS-REACTIVE GROUP-A STREPTOCOCCAL ANTI-DNA MONOCLONAL-ANTIBODIES TO DECONDENSED HUMAN SPERM DNA

被引：1

作者：

DCRUZ, OJ

DUNN, TS

HAAS, GG

RESHEF, E

CUNNINGHAM, MW

机构：

[1] UNIV OKLAHOMA,HLTH SCI CTR,DEPT MOLEC MED & PATHOL,OKLAHOMA CITY,OK 73190

[2] UNIV OKLAHOMA,HLTH SCI CTR,DEPT MICROBIOL & IMMUNOL,OKLAHOMA CITY,OK 73190

来源：

IMMUNOLOGICAL INVESTIGATIONS | 1994年 / 23卷 / 4-5期

关键词：

D O I：

10.3109/08820139409066827

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Four murine anti-streptococcal monoclonal antibodies (mAbs) that cross-react with human DNA were evaluated by immunofluorescence flow cytometry for their reactivity with condensed and decondensed human sperm nuclei. All 4 anti-DNA mAbs reacted with condensed sperm nuclei. The reactivity of 3 of these mAbs with decondensed sperm nuclei was 13 to 177 times higher than that found with condensed nuclei. Under identical conditions, mAbs to cytoskeletal/cytocontractile proteins lacked reactivity with decondensed sperm nuclei. Binding of monoclonal anti-DNA antibodies to decondensed sperm nuclei was abolished by preincubation with double-stranded DNA. The preferential binding of anti-DNA antibodies to decondensed sperm DNA suggests the utility of decondensed sperm nuclei as the antigenic substrate for screening anti-DNA antibodies by flow cytometry.

引用

页码：323 / 335

页数：13

共 21 条

[1] IMMUNOLOGY OF DNA .3. CRITHIDIA-LUCILIAE, A SIMPLE SUBSTRATE FOR DETERMINATION OF ANTI-DSDNA WITH IMMUNOFLUORESCENCE TECHNIQUE [J].