AFFINITY CHROMATOGRAPHY OF PIG HEART FUMARASE

被引：3

作者：

CHAUDHURI, S

THOMAS, EW

机构：

来源：

BIOCHEMICAL JOURNAL | 1979年 / 177卷 / 01期

关键词：

D O I：

10.1042/bj1770115

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

2-(5'-Phenylpentyl)fumaric acid was shown to be a competitive inhibitor (Ki 0.5 mM) of pig heart fumarase. After nitration of the aromatic ring, reduction to the amine and diazotization, the acid was attached via azo linkages to a Sepharose 4B-tyramine matrix. The resulting adsorbent was used for the affinity chromatography of crude fumarase, purifications of approx. 20-fold being obtained by specific elution with 0.01 M-citrate.

引用

页码：115 / 119

页数：5

共 13 条

[1] NEW PURIFICATION PROCEDURE FOR FUMARASE BASED OF AFFINITY CHROMATOGRAPHY - ISOLATION AND CHARACTERIZATION OF PIG-LIVER FUMARASE [J].