ENZYMATIC DETERMINATION OF ACIDS IN WINE .I. MALIC-, LACTIC- AND CITRIC-ACID

The enzymatic determination of l-malate with MDH, l-lactate with LDH and citrate with citrate - lyase was carried out in more than 100 samples of white and red wine, after elimination of interfering substances, such as tans and colouring matters. The contents of l-malate and citrate agreed very well with the results, found by a fractionation method on silica gel. Comparing the content of total lactate, found by the latter method, with the results of enzymatic determination of l-lactate, it was obvious, that d- and l-compounds occur in a different ratio, but at high lactic acid concentrations, however, the level of the l-form is higher. © 1969 Springer-Verlag.