PREPARATIVE ISOELECTRIC-FOCUSING OF APOLIPOPROTEINS C AND E FROM HUMAN VERY LOW-DENSITY LIPOPROTEINS

被引：76

作者：

MARCEL, YL

BERGSETH, M

NESTRUCK, AC

机构：

[1] Institut de Recherches Cliniques de Montréal, Montréal, Que. H2W 1R7

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 573卷 / 01期

基金：

英国医学研究理事会;

关键词：

Apolipoprotein; Isoelectric focussing; VLDL;

D O I：

10.1016/0005-2760(79)90184-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of isoelectric focusing on a gel-stabilized layer for the separation of the Tris-urea-soluble apolipoproteins of very low density lipoproteins is described. This 1 step method allows the separation of most apolipoproteins, which were then analyzed and characterized. Apolipoproteins CII and CI were isolated as single protein bands with apparent pI [isoelectric point] of 5.0 and 6.5, respectively. Apolipoprotein CII was biologically active and could activate lipoprotein lipase. Apolipoprotein CIII was separated into several protein bands with pI ranging from 4.7-5.1 as a function of their number of sialic acid residues. Apolipoprotein E was isolated and characterized into 5 polymorphic bands with pI of 5.7, 5.8, 5.9, 6.0 and 6.2, respectively.

引用

页码：175 / 183

页数：9

共 30 条

[1] ISOELECTRIC FRACTIONATION AND CHARACTERIZATION OF POLYPEPTIDES FROM HUMAN SERUM VERY LOW DENSITY LIPOPROTEINS [J].