DETERMINATION OF POLYAMINES IN RAT-TISSUES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER DERIVATIZATION WITH 1-PHENYLSULFONYL-3,3,3-TRIFLUOROPROPENE

A high performance liquid chromatographic method for polyamines using l-phenylsulfonyl-3,3,3-trifluoropropene as a precolumn derivatization reagent for UV detection has been developed. Polyamines were derivatized with 1-phenylsulfonyl-3,3,3-trifluoropropene by addition reaction in the presence of sodium carbonate under mild conditions to convert the corresponding UV-absorbing compounds quantitatively. The derivatized polyamines were separated on a reversed-phase column, Shim-pack CLC-ODS, with aqueous methanol-acetonitrile used as an eluent. Secondary amines like diethylamine and diisopropylamine did not react with this reagent. The detection limits for spermidine, putrescine, cadaverine and spermine at a signal-to-noise ratio of 3, were 0.3, 0.4, 0.4 and 0.6 pmol on column, respectively. The derivatization procedure was successfully applied to the determination of the polyamines in rat tissues. © 1990, The Japan Society for Analytical Chemistry. All rights reserved.