DIPHOSPHORYL LIPID-A DERIVED FROM LIPOPOLYSACCHARIDE (LPS) OF RHODOPSEUDOMONAS-SPHAEROIDES INHIBITS ACTIVATION OF 70Z/3 CELLS BY LPS

被引:56
作者
KIRKLAND, TN
QURESHI, N
TAKAYAMA, K
机构
[1] UNIV CALIF SAN DIEGO,DEPT MED,LA JOLLA,CA 92093
[2] VET ADM MED CTR,SAN DIEGO,CA 92161
[3] UNIV WISCONSIN,COLL AGR & LIFE SCI,DEPT BACTERIOL,MADISON,WI 53706
[4] WILLIAM S MIDDLETON MEM VET ADM MED CTR,MYCOBACTERIOL LAB,MADISON,WI 53705
关键词
D O I
10.1128/IAI.59.1.131-136.1991
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Diphosphoryl lipid A derived from nontoxic lipopolysaccharide (LPS) of Rhodopseudomonas sphaeroides ATCC 17023 did not stimulate the murine pre-B cell line 70Z/3 to synthesize surface immunoglobulin or kappa mRNA. However, it effectively blocked Escherichia coli LPS-induced activation of 70Z/3 cells in a concentration-dependent manner. This inhibition was specific only to cells activated by LPS, since it did not inhibit activation of 70Z/3 cells by gamma interferon. Maximal inhibitory effect occurred when the antagonist was added within 2 h before adding the LPS. These results strongly suggested that R. sphaeroides diphosphoryl lipid A is competing with E. coli LPS for physiological lipid A receptors on the 70Z/3 cells.
引用
收藏
页码:131 / 136
页数:6
相关论文
共 26 条