VISUALIZATION OF REPLICATING BANDS IN PLANT CHROMOSOMES WITH A MONOCLONAL ANTI-BRDU ANTIBODY METHOD

被引：8

作者：

TANIGUCHI, K

TANAKA, R

机构：

[1] Laboratory of Plant Chromosome and Gene Stock, Faculty of Science, Hiroshima University

来源：

JAPANESE JOURNAL OF GENETICS | 1991年 / 66卷 / 04期

关键词：

D O I：

10.1266/jjg.66.485

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In order to detect DNA replication in chromosomes of four plant species, Allium fistulosum (2n = 16), Crepis capillaris (2n = 6), Haplopappus gracilis (2n = 4) and Chrysanthemum coronarium var. spatiosum (2n = 18), an immunological technique using monoclonal anti-BrdU antibody was utilized. In this technique, HCl was used for denaturation and avidin-biotin complex for the immunological process. The replicating bands which appeared with this technique were of unusually high resolution.

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页码：485 / 489

页数：5

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