PROPERTIES OF HIV MEMBRANE RECONSTITUTED FROM ITS RECOMBINANT GP160 ENVELOPE GLYCOPROTEIN

被引：5

作者：

CORNET, B ^{[1
]}

DECROLY, E ^{[1
]}

THINESSEMPOUX, D ^{[1
]}

RUYSSCHAERT, JM ^{[1
]}

VANDENBRANDEN, M ^{[1
]}

机构：

[1] UNIV CATHOLIQUE LOUVAIN,BIOL CELLULAIRE LAB,B-1348 LOUVAIN,BELGIUM

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 1992年 / 8卷 / 10期

关键词：

D O I：

10.1089/aid.1992.8.1823

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Human immunodeficiency virus (HIV) membrane has been reconstituted from the recombinant envelope glycoprotein precursor (gp160) by a detergent dialysis technique. Electron microscopy shows that gp160-virosomes are spherical vesicles with a mean diameter identical to that of viral particles. Enzyme-linked immunosorbent assay and immunogold labeling demonstrate efficient association of gp160 with lipid vesicles and proteolysis treatment reveals an asymmetric insertion with about 90% of glycoproteins having their gp120-moiety pointing outside. Glycoproteins are organized as dimers and tetramers and gp160 retains its ability to specifically bind CD4 receptor after reconstitution into virosome.

引用

页码：1823 / 1831

页数：9

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