PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE-PEROXIDASE - FULL-LENGTH PIG BLASTOCYST CDNA SEQUENCE AND REGULATION BY SELENIUM STATUS

被引：61

作者：

SUNDE, RA ^{[1
]}

DYER, JA ^{[1
]}

MORAN, TV ^{[1
]}

EVENSON, JK ^{[1
]}

SUGIMOTO, M ^{[1
]}

机构：

[1] UNIV MISSOURI,COLL AGR FOOD & NAT RESOURCES,DEPT BIOCHEM,COLUMBIA,MO 65211

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 193卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1711

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated and sequenced an apparent full-length cDNA clone for phospholipid hydroperoxide glutathione peroxidase (Genbank accession number L12743) from a pig blastocyst cDNA library. The sequence encodes a polypeptide of 170 amino acids, including a TGA-encoded selenocysteine at residue 46, with a calculated M(r) of 19,492 Da. Use of this clone in Northern blot analysis of Se-deficient rat liver revealed that phospholipid hydroperoxide glutathione peroxidase mRNA levels were little affected by Se deficiency, whereas classicial glutathione peroxidase mRNA levels were decreased by 90% in the same samples. Lastly, liver phospholipid hydroperoxide glutathione peroxidase mRNA levels were not elevated in female rats, in contrast to classical glutathione peroxidase. © 1993 Academic Press, Inc.

引用

页码：905 / 911

页数：7

共 15 条

[1] Ausubel F, 1988, CURRENT PROTOCOLS MO
[2] NUCLEOTIDE-SEQUENCE OF A RAT GLUTATHIONE-PEROXIDASE CDNA
HO, YS
HOWARD, AJ
CRAPO, JD
[J]. NUCLEIC ACIDS RESEARCH, 1988, 16 (11) : 5207 - 5207
[3] THE EFFECT OF PROGRESSIVE SELENIUM DEFICIENCY ON ANTI-GLUTATHIONE PEROXIDASE ANTIBODY REACTIVE PROTEIN IN RAT-LIVER
KNIGHT, SAB
SUNDE, RA
[J]. JOURNAL OF NUTRITION, 1987, 117 (04) : 732 - 738
[4] POINT MUTATIONS DEFINE A SEQUENCE FLANKING THE AUG INITIATOR CODON THAT MODULATES TRANSLATION BY EUKARYOTIC RIBOSOMES
KOZAK, M
[J]. CELL, 1986, 44 (02) : 283 - 292
[5] GLUTATHIONE PEROXIDASE-ACTIVITY IN RAT LENS AND OTHER TISSUES IN RELATION TO DIETARY SELENIUM INTAKE
LAWRENCE, RA
SUNDE, RA
SCHWARTZ, GL
HOEKSTRA, WG
[J]. EXPERIMENTAL EYE RESEARCH, 1974, 18 (06) : 563 - 569
[6] MAIORINO M, 1990, METHOD ENZYMOL, V186, P448
[7] MCKOWN DM, 1978, J RADIOANAL CHEM, V43, P409
[8] PROHASKA JR, 1993, IN PRESS COMP BIOC B
[9] EFFECT OF SELENIUM STATUS ON MESSENGER-RNA LEVELS FOR GLUTATHIONE-PEROXIDASE IN RAT-LIVER
SAEDI, MS
SMITH, CG
FRAMPTON, J
CHAMBERS, I
HARRISON, PR
SUNDE, RA
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 153 (02) : 855 - 861
[10] PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE-PEROXIDASE IS A SELENO-ENZYME DISTINCT FROM THE CLASSICAL GLUTATHIONE-PEROXIDASE AS EVIDENT FROM CDNA AND AMINO-ACID SEQUENCING
SCHUCKELT, R
BRIGELIUS-FLOHE, R
MAIORINO, M
ROVERI, A
REUMKENS, J
STRASSBURGER, W
URSINI, F
WOLF, B
FLOHE, L
[J]. FREE RADICAL RESEARCH COMMUNICATIONS, 1991, 14 (5-6): : 343 - 361

← 1 2 →