CLONING AND EXPRESSION OF A NOVEL MOUSE SOMATOSTATIN RECEPTOR (SSTR2B)

被引：263

作者：

VANETTI, M ^{[1
]}

KOUBA, M ^{[1
]}

WANG, XM ^{[1
]}

VOGT, G ^{[1
]}

HOLLT, V ^{[1
]}

机构：

[1] UNIV MUNICH,INST PHYSIOL,PETTENKOFERSTR 12,W-8000 MUNICH 2,GERMANY

来源：

FEBS LETTERS | 1992年 / 311卷 / 03期

关键词：

SOMATOSTATIN; RECEPTOR; MOUSE; ALTERNATE SPLICING; EXPRESSION; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0014-5793(92)81122-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A mouse somatostatin (SS) receptor cDNA was cloned from neuroblastoma x glioma (NG108-15) cells. The sequence is almost identical to that of the mouse SSTR2 receptor [(1992) Proc. Natl. Acad. Sci. USA 89, 251)] but lacks about 300 nucleotides between transmembrane domain VII and the C-terminus. This spliced variant of SSTR2 (designated SSTR2B) encodes a protein which is 23 residues shorter than that predicted from the SSTR2 sequence, and differs in 15 amino acids at the C-terminus. mRNA corresponding to SSTR2B occurs in mouse tissues in higher abundance than that of SSTR2. SSTR2B binds SS peptides with high affinity when expressed in mammalian cells.

引用

页码：290 / 294

页数：5

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