ISOLATION OF A PROTEIN COMPLEX FROM PURULENT SPUTUM CONSISTING OF PROTEINASE-3 AND ALPHA-1-ANTITRYPSIN REACTIVE WITH ANTINEUTROPHIL CYTOPLASMIC ANTIBODIES

被引：14

作者：

BALLIEUX, BEPB ^{[1
]}

HAGEN, EC ^{[1
]}

VANDERKEUR, C ^{[1
]}

ZEGERS, ND ^{[1
]}

VANES, LA ^{[1
]}

VANDERWOUDE, FJ ^{[1
]}

DAHA, MR ^{[1
]}

机构：

[1] TNO,MED BIOL LAB,RIJSWIJK,NETHERLANDS

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1993年 / 159卷 / 1-2期

关键词：

PROTEINASE-3; ALPHA-1-ANTITRYPSIN; ANTINEUTROPHIL CYTOPLASMIC ANTIBODY; PURULENT SPUTUM;

D O I：

10.1016/0022-1759(93)90142-T

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The detection of ANCA (anti-neutrophil cytoplasmic antibodies) is of importance in the diagnosis of Wegener's granulomatosis (WG) and solid-phase assays for the detection of c-ANCA have been set up by various groups, using purified proteinase-3 (PR-3) in an ELISA or RIA. For the isolation of PR-3 large numbers of PMNs are needed. We therefore examined the possibility of isolating PR-3 from the purulent sputum of patients with chronic bronchitis or cystic fibrosis, since large numbers of PMNs and their degranulation products are present in such material. By a three-step chromatographic procedure (4-phenylbutylamine affinity chromatography, Biorex 70 cation exchange chromatography and monoclonal antibody anti-PR-3 affinity chromatography) we isolated a 53 kDa protein that was recognized on immunoblot by MoAbs directed against PR-3 and alpha-antitrypsin (alpha1AT). We show that the 53 kDa protein is a complex of PR-3 and alpha1AT. This complex is reactive with a selected set of c-ANCA positive sera from patients with Wegener's granulomatosis

引用

页码：63 / 70

页数：8

共 26 条

[1] DOWN-REGULATION OF A SERINE PROTEASE, MYELOBLASTIN, CAUSES GROWTH ARREST AND DIFFERENTIATION OF PROMYELOCYTIC LEUKEMIA-CELLS [J].