RECENT ADVANCES IN DIFFERENTIAL DISPLAY

被引：227

作者：

LIANG, P

PARDEE, AB

机构：

[1] Division of Cell Growth and Regulation, Dana-Farber Cancer Institute, Boston, MA 02115

来源：

CURRENT OPINION IN IMMUNOLOGY | 1995年 / 7卷 / 02期

关键词：

D O I：

10.1016/0952-7915(95)80015-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Differential display and RNA arbitrary primed polmerase chain reaction are methods recently designed to identify and isolate differentially expressed genes. Methodological modifications have since been introduced to streamline the techniques. The major effort has centered on how to eliminate false positives as approached from a variety of angles, ranging from RNA sample preparation, northern blot confirmation, primer length variation, to better experimental design.

引用

页码：274 / 280

页数：7

共 41 条

[1]

AASHEIM HC, 1994, BIOTECHNIQUES, V16, P716

[2] IDENTIFICATION OF DIFFERENTIALLY EXPRESSED MESSENGER-RNA SPECIES BY AN IMPROVED DISPLAY TECHNIQUE (DDRT-PCR) [J].