CLONING OF LACI GENE INTO A COLE1 PLASMID

被引：20

作者：

HARE, DL

SADLER, JR

机构：

[1] Department of Biophysics and Genetics, University of Colorado Medical Center, Denver

来源：

GENE | 1978年 / 3卷 / 04期

基金：

美国国家卫生研究院;

关键词：

Lactose repressor overproduction; pMB9; plasmid; λh80dlac DNA;

D O I：

10.1016/0378-1119(78)90037-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The binding of lac repressor to lac operator was utilized to isolate an EcoRI fragment of λh80dlac (i+ as well as iq) that contains the lacI gene and lac promoter-operator regions. Ligation of this fragment into EcoRI cleaved pMB9 yielded chimeric DNA molecules of mol.w.t.9.6 · 106 and 1.5 · 107 daltons. Transformed strains containing the plasmids were analyzed for repressor production in vivo and in vitro. Repressor production in one plasmid strain is 7-fold greater than that in heat-inducible λh80dlac(iq lysogens. © 1978.

引用

页码：269 / 278

页数：10

共 19 条

[1]

BETZ JL, 1976, J MOL BIOL, V105, P293, DOI 10.1016/0022-2836(76)90113-3

[2]

Bourgeois S., 1970, LACTOSE OPERON, P325

[3] MOLECULAR-PARAMETERS CHARACTERIZING INTERACTION OF ESCHERICHIA-COLI LAC REPRESSOR WITH NON-OPERATOR DNA AND INDUCER [J].